Company Overview

Prime Medicine was founded to bring the promise of gene editing to patients. We use Prime Editing, a next-generation technology that can “search and replace” to restore normal genetic function almost anywhere in the genome. Prime Editing searches to find the precise place in the genome to edit and replaces the segment of faulty DNA with a correct copy of DNA.  A single Prime Editor can correct the individual mutations found across patients, meaning Prime Editing can potentially address more than 90 percent of known disease-causing genetic mutations.  

We envision a world where this technology can cure, halt, and ultimately prevent genetic diseases, providing lifelong benefit to patients.  Prime Medicine offers the opportunity to shape the future of gene editing and transform how medicines are used to treat disease. Realizing the promise of Prime Editing requires a talented team with diversity of viewpoints and expertise. We’re building a group of dedicated, scientifically curious individuals who are passionate about helping patients live longer, healthier lives.

Position Overview:

We are currently seeking an established leader in RNA Chemistry who is motivated, creative, has a track record of success in RNA chemistry innovation, building teams and people development and management, to join our growing group of Chemists at our new dedicated Chemistry facility

Deep expertise and leadership in solid phase oligonucleotide synthesis, purification and characterization using a range of nucleoside chemistries across different platforms (eg Mermade, AKTA, Dr Oligo) will be central to this role to oversee our RNA medicinal chemistry team, to generate long Prime Editor guide RNA to serve the needs of our early research organization and to invent and innovate with methods and techniques to improve yield, purity and potency.  In this position, you will play a key role in building out Prime’s RNA synthetic chemistry group mentor and train junior scientists, oversee and be responsible for the safety, operation of equipment and training of scientists, and work closely with Chemistry and R&D leadership. The successful candidate will demonstrate their network in the industry, a clear track record and an ability to both supervise and be hands-on when required. The ideal candidate will be comfortable working in a cross-functional role and excel as part of a diverse team.

Key Responsibilities:

  • Oversee, mentor and develop a group of chemists involved in developing, establishing, and evaluating solid-phase synthesis and downstream purification and analytical methods for guide RNAs
  • Responsible for Prime’s solid-phase synthesis of long-RNAs (>120-mers) on MerMade/Dr Oligo synthesizers to produce high quality RNAs with and without chemical modifications and robust evaluation of the quality of RNA synthesis and improve synthetic, purification, and analytical protocols
  • Actively evaluate incorporation of chemical modifications in RNA to identify opportunities for improving stability, safety and editing efficiency of guide RNAs
  • Provide technical guidance on design and chemical modifications for low to mid-scale synthesis of long-RNAs for in vitro and in vivo screening
  • Interface with Analytics, Process Development, Delivery, Pharmacology, Platform and Disease program teams
  • Oversee and approve protocols, SOPs, technical documents, and reports for synthesis lab
  • Review, mentor and oversee experimental design execution,
  • Deliver reproducible and impactful results under ambitious timelines
  • Understand the landscape of clinical and process materials, liaison with vendors, and maintain appropriate inventory
  • Oversee team’s electronic lab notebooks and documentation records,
  • Present and mentor presentations of findings to multi-disciplinary teams in various oral and written formats
  • Build safety requirements and procedures into daily working practice and maintain a culture of best practice

Qualifications:

  • PhD in Chemistry, Biochemistry, Biotechnology, or related field with no less than 8 years of industry experience with solid-phase synthesis. Academic experience may be considered.
  • Must demonstrate mastery of solid-phase oligonucleotide synthesis, purification/analytical anion-exchange/reverse-phase/size-exclusion chromatography techniques.
  • Demonstrated mastery of laboratory-scale automated DNA/RNA synthesizers (eg Mermade), including high throughput instruments (Dr Oligo synthesizers).
  • Experience in design and developing chemically modified RNA synthesis and purification protocols, based on structure-based design principles.
  • Demonstrated experience of conjugation chemistry conjugates including peptides, dyes, and other small and large molecules.
  • Strong understanding of biophysical and biochemical aspects of nucleic acids is desired.
  • Expertise with analysis of chemically modified DNA/RNA oligonucleotides, such as HPLC/UPLC, UV thermal melting, LC-UV-Fluor, LC-MS, and slab/capillary gel electrophoresis techniques is required.
  • Strong understanding of nucleic acid structure-activity relationships and safety profiles of nucleic acids and their modifications is desirable.
  • Highly self-motivated, driven, can-do, make it happen attitude with ability to own and drive projects.
  • Strong collaborator with cross-functional teams, external partners, CROs.
  • Embraces continuous learning, open-minded, curious, mentality, highly-organized, and detail-oriented.
  • Strong communication skills, presenting to leadership, chemistry department and cross functional teams.

Prime Medicine is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, age, sexual orientation, gender identity, disability, veteran status, or any other classification protected by law.

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