Prime Medicine was founded to bring the promise of gene editing to patients. We use Prime Editing, a next-generation technology that can “search and replace” to restore normal genetic function almost anywhere in the genome. Prime Editing searches to find the precise place in the genome to edit and replaces the segment of faulty DNA with a correct copy of DNA. A single Prime Editor can correct the individual mutations found across patients, meaning Prime Editing can potentially address more than 90 percent of known disease-causing genetic mutations.
We envision a world where this technology can cure, halt, and ultimately prevent genetic diseases, providing lifelong benefit to patients. Prime Medicine offers the opportunity to shape the future of gene editing and transform how medicines are used to treat disease. Realizing the promise of Prime Editing requires a talented team with diversity of viewpoints and expertise. We’re building a group of dedicated, scientifically curious individuals who are passionate about helping patients live longer, healthier lives.
We are currently seeking a motivated, creative, and experienced scientist with expertise in solid phase oligonucleotide synthesis to join our growing RNA medicinal chemistry and delivery team at our new dedicated chemistry facility. In this position, you will play a key role in building out Prime’s RNA synthetic chemistry group and set up new synthesis/purification workflows to produce high quality synthetic oligonucleotides for preclinical drug development. The successful candidate will have significant experience in an industrial setting, with hands-on experience in the operation of lab-scale oligonucleotide synthesizers. The ideal candidate will be comfortable working in a cross-functional role and excel as part of a diverse team.
- Develop, establish, and evaluate solid-phase synthesis and downstream purification and analytical methods for guide RNAs
- Execute solid-phase synthesis of long-RNAs (>120-mers) on MerMade/Dr Oligo synthesizers to produce high quality RNAs with and without chemical modifications
- Actively evaluate effectiveness of RNA synthesis and improve synthetic, purification, and analytical protocols
- Actively evaluate incorporation of chemical modifications in RNA to identify opportunities for improving stability, safety and editing efficiency of guide RNAs
- Provide technical guidance on design and chemical modifications for low to mid-scale synthesis of long-RNAs for in vitro and in vivo screening
- Involve in RNA conjugation chemistries and development of conjugates
- Interface with Analytics, Process Development, Delivery, Pharmacology, Platform and Disease program teams
- Author, review, and approve protocols, SOPs, technical documents, and reports for synthesis lab
- Independently plan and execute experiments, perform data analysis and interpretation
- Deliver reproducible and impactful results under ambitious timelines
- Understand the landscape of clinical and process materials, liaison with vendors, and maintain appropriate inventory
- Maintain thorough electronic lab notebooks and documentation records, and present findings to multi-disciplinary teams in various oral and written formats
- Adhere to all laboratory safety requirements and procedures, assist in maintaining general laboratory functionality, including lab equipment maintenance and the ordering, and receiving of lab supplies
- PhD in Chemistry, Biochemistry, Biotechnology, or related field with no less than 2-6 years of industry experience with solid-phase synthesis. Academic experience is considered.
- Must have hands on experience of solid-phase oligonucleotide synthesis, purification/analytical anion-exchange/reverse-phase/size-exclusion chromatography techniques.
- Demonstrated competency in operating laboratory-scale automated DNA/RNA synthesizers (MerMades), including high throughput instruments (Dr Oligo synthesizers).
- Demonstrated competency and hands on experience are required in designing and developing chemically modified RNA synthesis and purification protocols.
- Demonstrated experience of designing and synthesis of oligonucleotide conjugates with peptides, dyes, and other small and large molecules.
- Experience with chemical and enzymatic ligations of nucleic acids is advantageous.
- Strong understanding of biophysical and biochemical aspects of nucleic acids is desired.
- Demonstrated hands on experience with analysis of chemically modified DNA/RNA oligonucleotides, such as HPLC/UPLC, UV thermal melting, LC-UV-Fluor, LC-MS, and slab/capillary gel electrophoresis techniques is required.
- Strong understanding of nucleic acid structure-activity relationships and safety profiles of nucleic acids and their modifications is desirable.
- Highly self-motivated, driven, can-do, make it happen attitude with ability to own and drive projects.
- Strong collaborator with cross-functional teams and external partners.
- Capable of continuous learning, open-minded, curious, highly organized, and detail-oriented.
- Ability to work with CROs, strong computer, presentation, and communications skills are a must.
Prime Medicine is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, age, sexual orientation, gender identity, disability, veteran status, or any other classification protected by law.