Prime Medicine was founded to bring the promise of gene editing to patients. We use Prime Editing, a next-generation technology that can “search and replace” to restore normal genetic function almost anywhere in the genome. Prime Editing searches to find the precise place in the genome to edit and replaces the segment of faulty DNA with a correct copy of DNA. A single Prime Editor can correct the individual mutations found across patients, meaning Prime Editing can potentially address more than 90 percent of known disease-causing genetic mutations.
We envision a world where this technology can cure, halt, and ultimately prevent genetic diseases, providing lifelong benefit to patients. Prime Medicine offers the opportunity to shape the future of gene editing and transform how medicines are used to treat disease. Realizing the promise of Prime Editing requires a talented team with diversity of viewpoints and expertise. We’re building a group of dedicated, scientifically curious individuals who are passionate about helping patients live longer, healthier lives.
We are currently seeking a motivated, creative, and experienced scientist with expertise in mRNA platform biology to join our growing team focused on RNA Technologies. The successful candidate will have significant experience with synthetic biology and biological mechanisms of therapeutic mRNA design and in vitro characterization. Experience with Ribosomal engagement and cell based mRNA half-life and expression assays is highly desirable. The ideal candidate will be proactive, enthusiastic, collaborative, and demonstrate good communication, with a desire to leverage in vitro characterization methods to improve mRNA stability, potency, and delivery.
- Develop and execute protocols that investigate ribosomal engagement of Prime Editor mRNAs including ribosomal profiling and ribosomal footprinting in a variety of cell types to evaluate and inform mRNA design and optimization
- Independently plan and perform assays that investigate Prime Editor mRNA stability and protein translation levels in cell free and cell-based assays.
- Characterize RNA-protein interactions key to mRNA metabolism and function in cellular and cell-free contexts
- Synthesize mRNA by in vitro transcription (IVT), and develop novel methodologies including both chemical and enzymatic reactions to incorporate chemical modifications in collaboration with mRNA chemistry team and test them in cell free and cell based assays
- Perform data analysis and interpretation and present findings in both small team and company-wide settings
- Collaborate cross functionally across the organization including RNA design, automation, screening, pharmacology, and program teams to characterize mRNAs and support program advancement in pre-clinical settings.
- Maintain thorough electronic lab notebooks and documentation records
- Adhere to all laboratory safety requirements and procedures, assist in maintaining general laboratory functionality, including lab equipment maintenance and the ordering, and receiving of lab supplies
- Create and write reports and SOPs to support development and regulatory filings
- PhD in Cellular or molecular biology, Synthetic biology, chemical biology, Biochemistry, or related disciplines with 3+ years of industry experience preferred.
- Experience in cell-based assay development that investigate therapeutic mRNA characteristics such as ELISA based protein translation assays, mRNA stability, and ribosomal engagement.
- Direct hands-on experience with ribosomal profiling and/or ribosomal footprinting, RT-qPCR, and protein ELISAs are highly preferred
- Significant experience in cell culture and nucleic acid transfections of immortalized and/or primary cell lines including execution and analysis of end point and kinetic dose response curves to evaluate mRNA stability and potency
- Strong knowledge of nucleic acid molecular biology
- Knowledge and preferably hands on experience of mRNA synthesis by invitro transcription (IVT), purification, analysis, and delivery is preferred
- Experience in chemical and enzymatic methods for modifying RNA is a plus
- Thorough knowledge of mRNA 5’-cap, open reading frame, UTR, and polyA tail optimization and design principles
- Experience leading and collaborating cross-functionally with chemistry, Biology, pharmacology, and program team partners
- Capable of continuous learning, open-minded, curious, highly organized, and detail-oriented with excellent written and verbal communication skills
Prime Medicine is proud to be an equal opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, national origin, age, sexual orientation, gender identity, disability, veteran status, or any other classification protected by law.